Most likely has to do with the way the round is feeding.
Common with 1st round flyers, in many .22's because the first round is loaded from an open bolt, and the subsequent rounds from a closed bolt.
Has to do with the feeding being slightly different, and the inconsistency causes the 2 rounds to print differently.
Solution is to load the mag from a closed bolt, and worked in both my 10/22 target, and Savage MKII to improve groupings.
Might be a similar issue with the last round feeding differently, causing bullet setback, deformation, scratching, etc.