ES first then seating depth?

In my experience nodes or whatever you want to call them are a real repeatable thing. YMMV and all that. There are other variables that have a bigger impact (powder lots, or reloading process, etc) that can mask it but it is there. I agree though that doing a ladder with 1 shot per charge isn't going to show you exactly where a node is. A ladder will usually show you areas where velocity is not stable though, even with those single measurements. Increasing the sample size won't make unstable areas more stable. At a certain point you need to decide what your goals are and what's good enough. You can burn up a barrel and spend a fortune on components easy enough chasing statistical significance with large sample sizes and it may not matter for your needs. A ladder test is just another piece of information. Use it or not. That's all part of the fun in figuring this stuff out.
 
Certain calibers, with certain bullet weights, with certain barrel lengths, etc will have known accuracy areas within a certain fps. Even between different barrels. Especially when the same barrel and reamers are used. Go look at the latest F class John video on YouTube, he runs his known ammo through a different barrel ( adjusting tuner ) and the results are pretty dang good. Certainly close enough for just a tweak to be spot on. It seems most long range target shooters adjust loads at each event anyway, sometimes its good from the start, other times it’s a quick seating change, ( they load long, to be able to adjust seating.) or a change of powder, or a twist of the tuner. My 6.5 is happy at 2710fps, 140 grain bullets, with 41.4 grains of H4350. Even when I open a new non matching pound of powder. If it eventually stops shooting well, it’s time to adjust seating depth 0.007”, and use the same everything else and it’s back to normal. I don’t shoot 100 shot per charge weight OCW tests and 100 shot per bullet seating change in tests but trends can be found with 3 shot groups and confirmed and often slightly adjusted at distance.
 
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