In my experience nodes or whatever you want to call them are a real repeatable thing. YMMV and all that. There are other variables that have a bigger impact (powder lots, or reloading process, etc) that can mask it but it is there. I agree though that doing a ladder with 1 shot per charge isn't going to show you exactly where a node is. A ladder will usually show you areas where velocity is not stable though, even with those single measurements. Increasing the sample size won't make unstable areas more stable. At a certain point you need to decide what your goals are and what's good enough. You can burn up a barrel and spend a fortune on components easy enough chasing statistical significance with large sample sizes and it may not matter for your needs. A ladder test is just another piece of information. Use it or not. That's all part of the fun in figuring this stuff out.